Gaussian Aquila optimizer based dual convolutional neural networks for identification and grading of osteoarthritis using knee joint images.

Degenerative musculoskeletal disease known as Osteoarthritis (OA) causes serious pain and abnormalities for humans and on detecting at an early stage, timely treatment shall be initiated to the patients at the earliest to overcome this pain. In this research study, X-ray images are captured from the humans and the proposed Gaussian Aquila Optimizer based Dual Convolutional Neural Networks is employed for detecting and classifying the osteoarthritis patients. The new Gaussian Aquila Optimizer (GAO) is devised to include Gaussian mutation at the exploitation stage of Aquila optimizer, which results in attaining the best global optimal value. Novel Dual Convolutional Neural Network (DCNN) is devised to balance the convolutional layers in each convolutional model and the weight and bias parameters of the new DCNN model are optimized using the developed GAO. The novelty of the proposed work lies in evolving a new optimizer, Gaussian Aquila Optimizer for parameter optimization of the devised DCNN model and the new DCNN model is structured to minimize the computational burden incurred in spite of it possessing dual layers but with minimal number of layers. The knee dataset comprises of total 2283 knee images, out of which 1267 are normal knee images and 1016 are the osteoarthritis images with an image of 512 × 512-pixel width and height respectively. The proposed novel GAO-DCNN system attains the classification results of 98.25% of sensitivity, 98.93% of specificity and 98.77% of classification accuracy for abnormal knee case-knee joint images. Experimental simulation results carried out confirms the superiority of the developed hybrid GAO-DCNN over the existing deep learning neural models form previous literature studies.

Mercury in the feathers of Golden eagle (Aquila chrysaetos) from Western Carpathian, Slovakia.

In this study, mercury (Hg) concentrations were detected in feathers of golden eagle (Aquila chrysaetos), a bird that typically inhabits alpine and forest areas. The mercury rates in feathers were compared in two groups of eagles: first, estimated home range (breeding, hunting, etc.) was located only in forest and lowland meadow habitats; second, the home range also included alpine habitats-rocks and meadows. Consequently, mercury concentration based on the feather typology were observed and the mercury levels in feathers were also compared among different Slovak Western Carpathian districts. It was found that there was no significant difference between groups classified by elevation level, which we attribute to the fact that eagle hunting territories are broad, so that alpine-dwelling and forest-dwelling eagles do not only reflect the pollution of the environments they typically inhabit. Non-significant differences were found also within different feather types, which means that the type of feather is not crucial for tracking mercury in eagle feathers. As the measurement of feather appears to be a simple and non-invasive method, the detection of non-significant differences in diverse types of golden eagle feathers provides useful knowledge for the future environment monitoring. The average mercury concentration measured in eagle samples was lower than the mercury concentration causing health complications among birds of prey. Our assumption that due to past mining activity in the Spis region, the highest concentration in this region would be observed was confirmed.

Diagnosis and Surgical Treatment of a Renal Cyst Causing Unilateral Lameness in a Golden Eagle (Aquila chrysaetos).

This report describes the diagnosis and treatment of a benign renal cyst in an adult, female golden eagle (Aquila chrysaetos) presented for unilateral leg lameness. A cyst at the cranial division of the left kidney was diagnosed by computed tomography and was suspected of compressing the lumbosacral nerve plexus, resulting in limb lameness. The renal cyst was incompletely excised because the cyst wall was closely adhered to the kidney parenchyma and local blood supply. Fluid analysis and surgical biopsy of the cyst and left kidney confirmed the diagnosis of a benign renal cyst. No evidence of an infectious, inflammatory, or neoplastic etiology was noted. Postoperatively, the eagle's lameness resolved and the bird was ultimately released following recovery. During treatment for the renal cyst, the eagle was concurrently found to have increased serum titers on elementary body agglutination for Chlamydia psittaci and a positive titer for Aspergillus species antibody testing. The bird was administered doxycycline, azithromycin, and voriconazole for treatment of these potential pathogens prior to release. Unfortunately, the eagle was found dead 86 days postrelease due to an unknown cause. To the authors' knowledge, this is the first report of a golden eagle with a benign solitary renal cyst causing unilateral lameness secondary to nerve compression that was resolved with surgical excision.

Accumulation of microplastics in predatory birds near a densely populated urban area.

The pollution due to plastic and other anthropogenic particles has steadily increased over the last few decades, presenting a significant threat to the environment and organisms, including avian species. This research aimed to investigate the occurrence of anthropogenic pollutants in the digestive and respiratory systems of four birds of prey: Common Buzzard (Buteo buteo), Black Kite (Milvus migrans), Eurasian Sparrowhawk (Accipiter nisus), and Northern Goshawk (Accipiter gentilis). The results revealed widespread contamination in all species with microplastics (MPs) and cellulosic anthropogenic fibers (AFs), with an average of 7.9 MPs and 9.2 AFs per specimen. Every digestive system contained at least one MP, while 65 % of specimens exhibited MPs in their respiratory systems. This is the work reporting a high incidence of MPs in the respiratory system of birds, clearly indicating inhalation as a pathway for exposure to plastic pollution. The content of MPs and AFs varied significantly when comparing specimens collected from central Madrid with those recovered from other parts of the region, including rural environments, suburban areas, or less populated cities. This result aligns with the assumption that anthropogenic particles disperse from urban centers to surrounding areas. Additionally, the dominant particle shape consisted of small-sized fibers (> 98 %), primarily composed of polyester, polyethylene, acrylic materials, and cellulose fibers exhibiting indicators of industrial treatment. These findings emphasize the necessity for further research on the impact of plastic and other anthropogenic material contamination in avian species, calling for effective strategies to mitigate plastic pollution.

Neurological examination of clinically healthy pigeons (Columba livia domestica), mute swans (Cygnus olor), common buzzards (Buteo buteo), common kestrels (Falco tinnunculus) and northern goshawks (Accipiter gentilis).

BACKGROUND: A neurological examination is essential for determining the localisation of neurological lesions. However, in avian species, quantitative data regarding the practicability and feasibility of neurological tests are very limited. Therefore, the aim of this study was to establish normative data for the neurological examination of clinically healthy birds of different species. METHODS: Forty-two domestic and feral pigeons (Columba livia domestica), 42 mute swans (Cygnus olor), 12 common buzzards (Buteo buteo), 24 common kestrels (Falco tinnunculus) and six northern goshawks (Accipiter gentilis) were examined. All birds underwent a predefined neurological examination. Interobserver variations between three examiners were investigated in 11 pigeons and 11 mute swans. RESULTS: All postural reaction tests, except for the drop and flap reaction in mute swans, provoked a consistent response in pigeons and mute swans, whereas postural reaction tests of the legs in raptors were often not performable. Cranial nerve tests and most of the spinal reflexes revealed variable responses in all birds. The gastrocnemius reflex was not provokable in any bird. Interobserver agreement was almost perfect (Gwet's AC1 coefficient ≥0.81) for 16 of 21 parameters in the examination in pigeons and for 14 of 21 in mute swans. LIMITATIONS: The inclusion of free-ranging birds, which were not used to handling and for which limited information regarding age, history of previous diseases, etc. was available, may have influenced the results. CONCLUSION: The normative neurological examination data provided in this study will help improve clinicians' interpretation of neurological examination results in the respective bird species.

Lesions and viral antigen distribution in bald eagles, red-tailed hawks, and great horned owls naturally infected with H5N1 clade 2.3.4.4b highly pathogenic avian influenza virus.

An epidemic of highly pathogenic avian influenza (HPAI) began in North America in the winter of 2021. The introduced Eurasian H5N1 clade 2.3.4.4b virus subsequently reassorted with North American avian influenza strains. This postmortem study describes the lesions and influenza A virus antigen distribution in 3 species of raptors, including bald eagles (Haliaeetus leucocephalus, n = 6), red-tailed hawks (Buteo jamaicensis, n = 9), and great horned owls (Bubo virginianus, n = 8), naturally infected with this virus strain based on positive reverse transcriptase polymerase chain reaction and sequencing results from oropharyngeal swabs. The birds presented with severe neurologic signs and either died or were euthanized because of the severity of their clinical signs and suspected influenza virus infection. Gross lesions were uncommon and included forebrain hemorrhages in 2 eagles, myocarditis in 1 hawk, and multifocal pancreatic necrosis in 3 owls. Histological lesions were common and included encephalitis, myocarditis, multifocal pancreas necrosis, multifocal adrenal necrosis, histiocytic splenitis, and anterior uveitis in decreasing frequency. Influenza A viral antigen was detected in brain, heart, pancreas, adrenal gland, kidney, spleen, liver, and eye. In conclusion, bald eagles, red-tailed hawks, and great horned owls infected with the HPAI clade 2.3.4.4b virus strain and showing neurological signs of illness may develop severe or fatal disease with histologically detectable lesions in the brain that are frequently positive for viral antigen.

Predicting the spatial distribution of wintering golden eagles to inform full annual cycle conservation in western North America.

Wildlife conservation strategies focused on one season or population segment may fail to adequately protect populations, especially when a species' habitat preferences vary among seasons, age-classes, geographic regions, or other factors. Conservation of golden eagles (Aquila chrysaetos) is an example of such a complex scenario, in which the distribution, habitat use, and migratory strategies of this species of conservation concern vary by age-class, reproductive status, region, and season. Nonetheless, research aimed at mapping priority use areas to inform management of golden eagles in western North America has typically focused on territory-holding adults during the breeding period, largely to the exclusion of other seasons and life-history groups. To support population-wide conservation planning across the full annual cycle for golden eagles, we developed a distribution model for individuals in a season not typically evaluated-winter-and in an area of the interior western U.S. that is a high priority for conservation of the species. We used a large GPS-telemetry dataset and library of environmental variables to develop a machine-learning model to predict spatial variation in the relative intensity of use by golden eagles during winter in Wyoming, USA, and surrounding ecoregions. Based on a rigorous series of evaluations including cross-validation, withheld and independent data, our winter-season model accurately predicted spatial variation in intensity of use by multiple age- and life-history groups of eagles not associated with nesting territories (i.e., all age classes of long-distance migrants, and resident non-adults and adult "floaters", and movements of adult territory holders and their offspring outside their breeding territories). Important predictors in the model were wind and uplift (40.2% contribution), vegetation and landcover (27.9%), topography (14%), climate and weather (9.4%), and ecoregion (8.7%). Predicted areas of high-use winter habitat had relatively low spatial overlap with nesting habitat, suggesting a conservation strategy targeting high-use areas for one season would capture as much as half and as little as one quarter of high-use areas for the other season. The majority of predicted high-use habitat (top 10% quantile) occurred on private lands (55%); lands managed by states and the Bureau of Land Management (BLM) had a lower amount (33%), but higher concentration of high-use habitat than expected for their area (1.5-1.6x). These results will enable those involved in conservation and management of golden eagles in our study region to incorporate spatial prioritization of wintering habitat into their existing regulatory processes, land-use planning tasks, and conservation actions.

A "One Arrow Three Eagle" Strategy to Improve CM-272 Primed Bladder Cancer Immunotherapy.

Immunotherapy using an immune-checkpoint blockade has significantly improved its therapeutic effects. CM-272, which is a novel epigenetic inhibitor of G9a, induces immunogenic cell death (ICD) for recovering the sensitivity to anti-PD-1 antibodies; however, the efficacy of CM-272 is greatly limited by promoting the transcription activity of HIF-1α to form a hypoxic environment. Here, a Fe3+ -based nanoscale metal-organic framework (MIL-53) is used to load CM-272 (ultra-high loading rate of 56.4%) for realizing an MIL-53@CM-272 nanoplatform. After entering bladder cancer cells, Fe3+ not only promotes the decomposition of H2 O2 into O2 for O2 -compensated sonodynamic therapy but reduces the high level of glutathione in the tumor microenvironment (TME) for enhancing reactive oxygen species, including ferroptosis and apoptosis. MIL-53 carriers can be degraded in response to the TME, accelerating the release of CM-272, which helps achieve the maximum effectiveness in an O2 -sufficient TME by attenuating drug resistance. Furthermore, MIL-53@CM-272 enhances dendritic cell maturation and synergistically combines it with an anti-programmed cell death protein 1 antibody during the study of immune-related pathways in the transcriptomes of bladder cancer cells using RNA-seq. This study presents the first instance of amalgamating nanomedicine with CM-272, inducing apoptosis, ferroptosis, and ICD to achieve the "one arrow three eagle" effect.

Taphonomy of harpy eagle predation on primates and other mammals.

The goal of this study is to provide a taphonomic analysis of bone fragments found in harpy eagle nests in the Brazilian Amazonia, utilizing the largest sample of prey remains collected to date. Harpy eagle kill samples were collected from nine nests, between June 2016 and December 2020 in Mato Grosso, Brazil. We identified the specimens, calculated the number of identified specimens (NISP) and minimum number of individuals (MNI). These metrics were used to estimate bone survivability and fragmentation. A total of 1661 specimens (NISP) were collected, representing a minimum number of 234 individuals (MNI). We identified at least nine species of primates, which represent 63.8% of the individuals in the kill sample. Harpy eagles preyed mostly on the medium-sized capuchin and bearded saki monkeys (28.2% of the MNI), and two-toed sloths (17.7% of the MNI). The large woolly monkeys also represented a significant portion of the sample (11.5% of the MNI). Three distinct patterns of bone survivability were found, one characterizing two-toed sloths, another characterizing medium-sized monkeys, and a third typical of woolly monkeys. We conclude that harpy eagle predation leaves an identifiable signature on the prey with a bone survivability pattern specific to each taxon. The intertaxon variations observed in the taphonomic signatures of harpy eagle kills should be taken into account when evaluating the potential influence of these raptors as accumulators of bone material in both paleontological and neontological assemblages.

Eagle jugular syndrome presenting with intracranial hypertension - Diagnosis and illustration of pathophysiology by multi-modality imaging.

Eagle jugular syndrome is an uncommon condition caused by compression of an elongated styloid process onto the internal jugular vein. Its presentation is non-specific but may represent in severe clinical consequences including venous thrombosis and intracranial haemorrhage. Thorough understanding of local anatomy is important in understanding the pathogenesis and establishing the diagnosis. Our case reported here illustrates the use of multimodality imaging, including dynamic Computer tomography manoeuvre, in identifying the site of obstruction and guidance towards successful surgical treatment.

A case of suspected chimpanzee scavenging in the Issa Valley, Tanzania.

Like humans, chimpanzees (Pan troglodytes) are well known for their vertebrate and invertebrate hunting, but they rarely scavenge. In contrast, while hunting and meat consumption became increasingly important during the evolution of the genus Homo, scavenging meat and marrow from carcasses of large mammals was also likely to be an important component of their subsistence strategies. Here, we describe a confrontational scavenging interaction between an adult male chimpanzee from the Issa Valley and a crowned eagle (Stephanoaetus coronatus), which resulted in the chimpanzee capturing and consuming the carcass of a juvenile bushbuck (Tragelaphus scriptus). We describe the interaction and contextualize this with previous scavenging observations from chimpanzees.

Surveys of eleven species of wild and zoo birds and feeding experiments in white-tailed eagles reveal differences in the composition of the avian gut microbiome based on dietary habits between and within species.

The composition of the gut microbiome varies due to dietary habits. We investigated influences of diet on the composition of the gut microbiome using the feces of 11 avian species, which consumed grain-, fish- and meat-based diets. We analyzed gut microbiome diversity and composition by next-generation sequencing (NGS) of 16S ribosomal RNA. The grain-diet group had higher gut microbiome diversity than the meat- and fish-diet group. The ratio of Bacteroidetes and Firmicutes phyla was higher in the grain-diet group than in the meat- and fish-diet groups. The grain-diet group had a higher ratio of Veillonellaceae than the meat-diet group and a higher ratio of Eubacteriaceae than the fish-diet habit group. To clarify the influence of diet within the same species, white-tailed eagles (Haliaeetus albicilla, n=6) were divided into two groups, and given only deer meat or fish for approximately one month. The composition of the gut microbiome of individuals in both groups were analyzed by NGS. There were indications of fluctuation in the levels of some bacteria (Lactobacillus, Coriobacteriales, etc.) in each diet group. Moreover, one individual for each group which switched each diet in last week changed to each feature of composition of bacterial flora. The above results show that the composition of the gut microbiome differ depending on diet, even within the same species.

Incidental eagle carcass detection can contribute to fatality estimation at operating wind energy facilities.

Risk of birds colliding with wind turbines, especially protected species like bald eagle and golden eagle in the U.S., is a fundamental wildlife challenge the wind industry faces when developing and operating projects. The U.S. Fish and Wildlife Service requires wind energy facilities that obtain eagle take permits document permit compliance through fatality monitoring. If trained Operations and Maintenance (O&M) staff can reliably detect and report carcasses during their normal routines, and their detection probability can be estimated, then their 'incidental detections' could contribute substantially towards demonstrating permit compliance. Our primary objective was to quantify incidental detection of eagle carcasses by O&M staff under a variety of landscape contexts and environmental conditions throughout a single year. We used the incidental detection probabilities, along with raptor carcass persistence data and area adjustments, to calculate overall probability of incidental detection (i.e., incidental g). We used feathered decoys as eagle-carcass surrogates for monthly detection trials at 6 study sites throughout the U.S. We evaluated the primary drivers of incidental detection using logit regression models including season, viewshed complexity, and a derived variable called the "density quartile" as covariates. We used an Evidence of Absence-based approach to estimate the overall probability of incidental detection. The incidental detection probabilities ranged from 0.28 to 0.78 (mean = 0.48). Detection probabilities decreased as viewshed complexity increased and as distance from the turbine increased. The resulting overall probability of incidental detection ranged from 0.07 to 0.47 (mean = 0.31). The primary drivers of variability in incidental g were detection probability and the area adjustment. Results of our research show that O&M staff were effective at detecting trial carcasses incidentally. Incorporating incidental detection in eagle fatality monitoring efforts is a reliable means of improving estimates of a facility's direct impacts on eagles.

Anomalous binocular vision in African Harrier-Hawks.

An animal's visual field is the three-dimensional space around its head from which it can extract visual information at any instant1. Bird visual fields vary markedly between species, and this variation is likely to be driven primarily by foraging ecology1,2,3. The binocular visual field is the region in which the visual fields of the two eyes overlap; thus, objects in the binocular field are imaged by both eyes simultaneously. The binocular field plays a pivotal role in the detection of symmetrical optic flow-fields, providing almost instantaneous information on the direction of travel and the time to contact a target towards which the head or feet is travelling; thus, information from the binocular field is crucial in guiding key foraging behaviours2,3. Here, we demonstrate an unusual visual field and binocular extent above the head in African Harrier-Hawks, also known as Gymnogenes (Polyboroides typus) compared to 18 other members of the Accipitridae4,5. We argue that the observed visual field can be attributed to the unusual and specific foraging behaviour of African Harrier-Hawks.

The prey of the Harpy Eagle in its last reproductive refuges in the Atlantic Forest.

The Harpy Eagle (Harpia harpyja) is threatened with extinction throughout its distribution in the neotropical forests. In the Atlantic Forest, deforestation has reduced the number of suitable habitats, with only a few remnant forest fragments hosting active nests; currently, the only known nests in this region are in the Central Atlantic Forest Ecological Corridor (CAFEC), in Brazil. Little is known about Harpy Eagle diets in this region, despite this information being essential for developing effective conservation strategies. We classified the composition, frequency, richness, ecological attributes, and conservation status of the species that make up the Harpy Eagle's diet in its last refuges in the CAFEC. Between 2017 and 2021, we collected and analyzed 152 prey remains and 285 camera trap photographs from seven active nests. We identified at least 16 mammal species (96.7%), one parrot and other bird remains (3.3%). The Harpy Eagle's diet consisted mainly of medium-sized arboreal, folivorous, frugivorous, and diurnal mammals. Five prey species are currently threatened with extinction at global, six at national and seven at regional levels. The majority of the diet consists of Sapajus robustus, which is threatened, and Bradypus variegatus, which is not threatened. In addition to the effects of habitat loss and hunting, the Harpy Eagle may also suffer from the decline in the populations of their prey in the Atlantic Forest.

More than just an eagle killer: The freshwater cyanobacterium Aetokthonos hydrillicola produces highly toxic dolastatin derivatives.

Cyanobacteria are infamous producers of toxins. While the toxic potential of planktonic cyanobacterial blooms is well documented, the ecosystem level effects of toxigenic benthic and epiphytic cyanobacteria are an understudied threat. The freshwater epiphytic cyanobacterium Aetokthonos hydrillicola has recently been shown to produce the "eagle killer" neurotoxin aetokthonotoxin (AETX) causing the fatal neurological disease vacuolar myelinopathy. The disease affects a wide array of wildlife in the southeastern United States, most notably waterfowl and birds of prey, including the bald eagle. In an assay for cytotoxicity, we found the crude extract of the cyanobacterium to be much more potent than pure AETX, prompting further investigation. Here, we describe the isolation and structure elucidation of the aetokthonostatins (AESTs), linear peptides belonging to the dolastatin compound family, featuring a unique modification of the C-terminal phenylalanine-derived moiety. Using immunofluorescence microscopy and molecular modeling, we confirmed that AEST potently impacts microtubule dynamics and can bind to tubulin in a similar matter as dolastatin 10. We also show that AEST inhibits reproduction of the nematode Caenorhabditis elegans. Bioinformatic analysis revealed the AEST biosynthetic gene cluster encoding a nonribosomal peptide synthetase/polyketide synthase accompanied by a unique tailoring machinery. The biosynthetic activity of a specific N-terminal methyltransferase was confirmed by in vitro biochemical studies, establishing a mechanistic link between the gene cluster and its product.

An elite approach to re-design Aquila optimizer for efficient AFR system control.

Controlling the air-fuel ratio system (AFR) in lean combustion spark-ignition engines is crucial for mitigating emissions and addressing climate change. In this regard, this study proposes an enhanced version of the Aquila optimizer (ImpAO) with a modified elite opposition-based learning technique to optimize the feedforward (FF) mechanism and proportional-integral (PI) controller parameters for AFR control. Simulation results demonstrate ImpAO's outstanding performance compared to state-of-the-art algorithms. It achieves a minimum cost function value of 0.6759, exhibiting robustness and stability with an average ± standard deviation range of 0.6823±0.0047. The Wilcoxon signed-rank test confirms highly significant differences (p<0.001) between ImpAO and other algorithms. ImpAO also outperforms competitors in terms of elapsed time, with an average of 43.6072 s per run. Transient response analysis reveals that ImpAO achieves a lower rise time of 1.1845 s, settling time of 3.0188 s, overshoot of 0.1679%, and peak time of 4.0371 s compared to alternative algorithms. The algorithm consistently achieves lower error-based cost function values, indicating more accurate control. ImpAO demonstrates superior capabilities in tracking the desired input signal compared to other algorithms. Comparative assessment with recent metaheuristic algorithms further confirms ImpAO's superior performance in terms of transient response metrics and error-based cost functions. In summary, the simulation results provide strong evidence of the exceptional performance and effectiveness of the proposed ImpAO algorithm. It establishes ImpAO as a reliable and superior solution for optimizing the FF mechanism-supported PI controller for the AFR system, surpassing state-of-the-art algorithms and recent metaheuristic optimizers.

A Modified Aquila-Based Optimized XGBoost Framework for Detecting Probable Seizure Status in Neonates.

Electroencephalography (EEG) is increasingly being used in pediatric neurology and provides opportunities to diagnose various brain illnesses more accurately and precisely. It is thought to be one of the most effective tools for identifying newborn seizures, especially in Neonatal Intensive Care Units (NICUs). However, EEG interpretation is time-consuming and requires specialists with extensive training. It can be challenging and time-consuming to distinguish between seizures since they might have a wide range of clinical characteristics and etiologies. Technological advancements such as the Machine Learning (ML) approach for the rapid and automated diagnosis of newborn seizures have increased in recent years. This work proposes a novel optimized ML framework to eradicate the constraints of conventional seizure detection techniques. Moreover, we modified a novel meta-heuristic optimization algorithm (MHOA), named Aquila Optimization (AO), to develop an optimized model to make our proposed framework more efficient and robust. To conduct a comparison-based study, we also examined the performance of our optimized model with that of other classifiers, including the Decision Tree (DT), Random Forest (RF), and Gradient Boosting Classifier (GBC). This framework was validated on a public dataset of Helsinki University Hospital, where EEG signals were collected from 79 neonates. Our proposed model acquired encouraging results showing a 93.38% Accuracy Score, 93.9% Area Under the Curve (AUC), 92.72% F1 score, 65.17% Kappa, 93.38% sensitivity, and 77.52% specificity. Thus, it outperforms most of the present shallow ML architectures by showing improvements in accuracy and AUC scores. We believe that these results indicate a major advance in the detection of newborn seizures, which will benefit the medical community by increasing the reliability of the detection process.

Per- and polyfluoroalkyl substances (PFAS) in white-tailed sea eagle eggs from Sweden: temporal trends (1969-2021), spatial variations, fluorine mass balance, and suspect screening.

Temporal and spatial trends of 15 per- and polyfluoroalkyl substances (PFAS) were determined in white-tailed sea eagle (WTSE) eggs (Haliaeetus albicilla) from two inland and two coastal regions of Sweden between 1969 and 2021. PFAS concentrations generally increased from ∼1969 to ∼1990s-2010 (depending on target and site) and thereafter plateaued or declined, with perfluorooctane sulfonamide (FOSA) and perfluorooctane sulfonate (PFOS) declining faster than most perfluoroalkyl carboxylic acids (PFCAs). The net result was a shift in the PFAS profile from PFOS-dominant in 1969-2010 to an increased prevalence of PFCAs over the last decade. Further, during the entire period higher PFAS concentrations were generally observed in coastal populations, possibly due to differences in diet and/or proximity to more densely populated areas. Fluorine mass balance determination in pooled samples from three of the regions (2019-2021) indicated that target PFAS accounted for the vast majority (i.e. 81-100%) of extractable organic fluorine (EOF). Nevertheless, high resolution mass-spectrometry-based suspect screening identified 55 suspects (31 at a confidence level [CL] of 1-3 and 24 at a CL of 4-5), of which 43 were substances not included in the targeted analysis. Semi-quantification of CL ≤ 2 suspects increased the identified EOF to >90% in coastal samples. In addition to showing the impact of PFAS regulation and phase-out initiatives, this study demonstrates that most extractable organofluorine in WTSE eggs is made up of known (legacy) PFAS, albeit with low levels of novel substances.

Molecular investigation of Haemoproteus and Plasmodium species of some raptors in Hatay province: new CytB lineages for raptors of Accipitriformes in Turkey.

Accipitriform raptors are significant indicators of biodiversity and environmental health. Currently, most of the studies on avian haemosporidian parasites are on passerine birds, and data on raptors is constricted, with similarities both around the world and in Turkey. This study aimed to investigate the presence of Haemoproteus and Plasmodium spp. in raptors by microscopy and nested PCR technique. The study material consisted of 47 accipitriform raptors (Buteo buteo: 14, Buteo rufinus: 7, Clanga pomarina: 8, Circaetus gallicus: 12, Milvus migrans: 6). The prevalence of haemosporidian infection was 12.8% (6/47, 1 from Buteo buteo, 4 from Clanga pomarina, 1 from Milvus migrans) microscopically and 14.9% (7/47) molecularly. One Circaetus gallicus, microscopically found to be negative, probably due to low parasitemia, was molecularly found to be positive. All PCR-positive amplicons were bidirectionally sequenced, and the identification of lineages of the isolates and phylogenetic analysis were performed using the MalAvi and GenBank databases. The study revealed H-MILANS02 lineage in Buteo buteo, H-MILANS02 and P-CIAE1 lineages in Clanga pomarina, P-GRW06 lineage in Circaetus gallicus, and P-RTSR1 lineage in Milvus migrans, respectively. While this study removes the uncertainty regarding the reporting of the H-MILANS02 lineage in Turkey, it is also the first report to reveal 3 different Plasmodium spp. CytB lineages in raptors. Moreover, the fact that the P-GRW06 lineage (Plasmodium elongatum) detected in passerine birds was detected in a raptor, Circaetus gallicus, draws attention to the need for further investigations on host-parasite interaction and gives clues about the host-shifting ability of this parasite.